Journal: Materials Today Bio

Article Title: Enhanced inhibition of neuronal ferroptosis and regulation of microglial polarization with multifunctional traditional Chinese medicine active ingredients-based selenium nanoparticles for treating spinal cord injury

doi: 10.1016/j.mtbio.2025.101758

Figure Lengend Snippet: TSIIA/TMP/APS@Se NPs regulate the polarization of BV2 cells and improve the inflammatory microenvironment to rescue PC12 cells in a co-culture system. (A–D) The ELISA quantitative analysis of the inflammatory cytokines in BV2 cells after different treatments. (E) Schematic illustration of the modulation of TSIIA/TMP/APS@Se NPs on BV2 polarization. (F) Protein expression images of iNOS and Arg-1 in BV2 cells after different treatment of nanoparticles. (G) Flow cytometry result of the BV2 cells to identify the M1 phenotype (F4/80, CD16/32 double positive) and M2 phenotype (F4/80, CD206 double positive) after incubation with F4/80, CD16/32, CD206. (H) Schematic illustration of a co-cultured system between PC12 cells and BV2 cells, indicating outcomes of diverse treatments with Se NPs. (I) The representative images of Live/Dead stains of PC12 cells after different treatments of the co-cultured system. Scale bar = 200 μm ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, and ns ( P > 0.05) suggested no statistical difference.

Article Snippet: The immortalized murine microglial cell line BV2 is commonly used as a surrogate for primary microglia in CNS research [ ].

Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Incubation, Cell Culture

Journal: Journal of Exercise Nutrition & Biochemistry

Article Title: Smallanthus sonchifolius leaf attenuates neuroinflammation

doi: 10.20463/jenb.2018.0014

Figure Lengend Snippet: Effects of ethanolic yacon leaf extract (YLE) on lipopolysaccharide (LPS)-induced BV2 microglial cell viability and the production of nitric oxide (NO). (A) BV2 cells were treated with YLE (10, 25, and 50 μg/mL) for 48 h and cell viability was examined using 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolim-5-carboxanilide (n = 4). Cell viability in the quiescent state is expressed as 100%. (B) Cells were co-treated with YLE and LPS (500 ng/mL) for 48 h. The production of NO in BV2 cells was determined using the Griess reagent (n = 6). Data are expressed as means ± SE. Values with the same letter are not significantly dfiferent, as determined byT ukey’s multiple range test (p < 0.05).

Article Snippet: BV2 cells were obtained from the Korean Cell Line Bank (Seoul, Korea) and grown in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C under 5% CO2.

Techniques:

Journal: Journal of Exercise Nutrition & Biochemistry

Article Title: Smallanthus sonchifolius leaf attenuates neuroinflammation

doi: 10.20463/jenb.2018.0014

Figure Lengend Snippet: Effects of ethanolic yacon leaf extract (YLE) on lipopolysaccharide (LPS)-induced mRNA expression of proinflammatory cytokines in BV2 cells. BV2 cells (2 × 105 cells) were seeded in 100 mm dishes for 24. Thhe cells were then incubated in the absence or presence of LPS (500 ng/mL) anYd LE (50 μg/mL) for 24 h. mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygensae (COX)-2, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α were assessed by the real-time polymerase chain reaction. (A–D) Relative mRNA expression compared with the LPS-treated group (100%). Data are expressed as means ± SE (n = 3). *Significant difference compared with LPS-only treatment (p < 0.05).

Article Snippet: BV2 cells were obtained from the Korean Cell Line Bank (Seoul, Korea) and grown in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C under 5% CO2.

Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction